Lipidomics, insertion of tail-anchored proteins into lipid bilayers, assembly of lipids into the double autophagosomal membrane.
NorthWest Building, Room 445.30
52 Oxford Street
Boston, MA 02115
Lab Size: Fewer than 5
The Denic lab takes advantage of novel methodologies for studying large-scale genetic interactions in budding yeast as well as mass spectrometry-based characterization of natively-isolated protein complexes in order to identify the essential components required for several membrane-associated cellular processes. We then carry out targeted and systematic biochemical reconstitution strategies using the identified components in order to go from parts lists to functional and mechanistic insights.
Denic V., Weissman J. S. (2007). A Molecular Caliper Mechanism for Determining Very Long-Chain Fatty Acid Length. Cell; 130, 663-67
Schuldiner M.,* Metz J., Schmid V., Denic V.* et al. (2008). The Get Complex Mediates Insertion of Tail-Anchored Proteins into the ER. Cell; 134, 634-45 (*co-first author)
Quan, E.M., Kamiya, Y., Kamiya, D., Denic, V., Weibezahn, J., Kato, K., Weissman, J.S. (2008). Defining the Glycan Destruction Signal for Endoplasmic Reticulum-Associated Degradation. Molecular Cell; 32, 870-877.
Jonikas, M. C., Collins, S. R., Denic, V., Oh, E., Quan, E. M., Schmid, V., Weibezahn, J., Schwappach, B., Walter, P., Weissman, J. S., Schuldiner, M. The Anatomy of a Cellular Folding Compartment. Science; in press. Science. 2009 Mar 27;323(5922):1693-7. PMID: 19325107